Device

Part:BBa_K817019:Design

Designed by: Shan Chi Hsieh   Group: iGEM12_NTU-Taida   (2012-09-18)


parAB operon from pseudomonas putida KT2440 chromosome


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1593
    Illegal XhoI site found at 519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1593
    Illegal NgoMIV site found at 98
    Illegal NgoMIV site found at 608
    Illegal NgoMIV site found at 1455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 822
    Illegal BsaI site found at 1173
    Illegal SapI.rc site found at 1227
    Illegal SapI.rc site found at 1335


Design Notes

This part have restriction site of EcoRI & PstI, mutagenesis is ongoing.


Source

pseudomonas putida KT2440 chromosome

References